La expresión del "gen temprano inmediato" c-fos es un marcador indirecto de la actividad neuronal porque c-fos suele expresarse cuando las neuronas disparan potenciales de acción. c-fos mRNA expression is correlated to glutamatergic neurotransmission and has been studied extensively in psychopharmacology. RNAscope (ACD Bio) is a patented in situ mRNA detection technology (1) that uses branched DNA amplification and z paired probes to deliver a robust and specific assay, designed primarily for use on formalin fixed paraffin sections. The technology, adapted to frozen brain tissue sections, allowed the study of the modulation of c-fos expresión en diferentes neurocircuitos por un antagonista D3 preferente, potencial antipsicótico, en ratones tratados con el bloqueador del canal del receptor de glutamato NMDA MK-801 (2). El método y su validación se describen en Cosi et al,https://doi.org/10.1016/j.mex.2021.101348
1] F. Wang, J. Flanagan, N. Su, L.-C. Wang, S. Bui, A. Nielson, X. Wu, H.-T. Vo, X.-J. Ma, Y. Luo, RNAscope®: A novel in situ RNA analysis platform for formalin-fixed paraffin-embedded tissues, J. Mol. Diagn. 14 (2012) 22-29, doi:10.1016/j.jmoldx.2011.08.002.
[2] C. Cosi, J.C. Martel, A.L. Auclair, G. Collo, L. Cavalleri, P. Heusler, L. Leriche, F. Gaudoux, P. Sokoloff, P.C. Moser, S. GattiMcArthur, Pharmacology Profile of F17464, a Dopamine D3 Antagonista preferente del receptor, Eur. J. Pharmacol. 890 (2021) 173635 https://doi.org/10.1016/j.ejphar.2020.173635.
Sobre el autor
Cristina Cosi, licenciado y doctorado, biólogo y farmacólogo, neurocientífico de formación, es consultor científico independiente en Kolabtree.
Ha dedicado su carrera a la comprensión de los mecanismos de la plasticidad neuronal y al descubrimiento de fármacos que puedan ser beneficiosos contra las enfermedades neurológicas (incluido el Parkinson) y neuropsiquiátricas y el dolor.
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